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KMID : 0665220090220010014
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Korean Journal of Food and Nutrition
2009 Volume.22 No. 1 p.14 ~ p.19
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Characterization of Lipase Produced from the Microorganisms Isolated from Mud-flat
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Choi Choong-Sik
Lee Soon-Youl
Lee Jae-Hag
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Abstract
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This study was performed to characterize the lipases produced from Gelidibacter sp. YH333 and Vibrio sp. YH339 isolated from mud flats for industrial application of a lipase. Amount of the lipases secreted from the isolated strains was sharply increased in the proportion of increase of number of the cells. The lipases produced from the isolated strains were constitutively secreted from the cells. The lipase activity of Gelidibacter sp. YH333 was higher than that of Vibrio sp. YH339 to p-nitrophenyl esters. The lipases produced from both strains showed the highest activity in p-nitrophenyl laulate among various p-nitrophenyl esters. The molecular weights of the lipases from Gelidibacter sp. YH333 were about 50 KDa and 25 KDa, respectively. Molecular weight of the lipase from Vibrio sp. YH339 was about 50 KDa.
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KEYWORD
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lipase, Vibrio, Gelidibacter, molecular weight, p-nitrophenyl laulate
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